| Registro completo |
| Provedor de dados: |
Ciência Rural
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| País: |
Brazil
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| Título: |
ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats
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| Autores: |
Oliveira,Karen Maciel de
Binda,Nancy Scardua
Lavor,Mário Sérgio Lima
Silva,Carla Maria Osório
Rosado,Isabel Rodrigues
Taguchi,Tatiana Malagoli
Alves,Endrigo Gabellini Leonel
Melo,Marília Martins
Gomez,Marcus Vinícius
Melo,Eliane Gonçalves de
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| Data: |
2016-01-01
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| Ano: |
2016
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| Palavras-chave: |
MVIIA
Cone snail
Cell viability
Glutamate
Reactive oxygen species
Lipid peroxidation
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| Resumo: |
This study aimed to investigate the neuroprotective effect of ω-conotoxin MVIIA (MVIIA) intralesional application in rats submitted to spinal cord injury. Male Wistar rats, weighing 300g±23.4, were distributed in five groups: negative control (SHAM), placebo (PLA), 5μM MVIIA, 10μM MVIIA and 20μM MVIIA MVIIA. After laminectomy of the 12th thoracic vertebra (SHAM), the PLA, 5μM MVIIA, 10μM MVIIA and 20μM MVIIA groups were subjected to acute compressive spinal cord trauma for five minutes, and then five minutes later, the animals received specific treatment in a standard total volume of 2µL, by intralesional route, using sterile PBS as placebo. Locomotor activity was assayed using Basso Beattie Bresnahan (BBB) scale to show the patterning of SCI. With 48 hours of injury, the animals were euthanized, the liquor sample was collected in atlantooccipital space, and also the spinal segment, including the epicenter and caudal region to injury. Assays were performed for mitochondrial viability, serum glutamate, production of reactive oxygen species (ROS) and lipid peroxidation (LP) were performed. The study design was randomized and the data submitted to ANOVA and comparison of means by SNK test, and data from BBB scale were evaluated using Kruskal-Wallis test (P<0.05). There was no significant difference between groups in BBB scores. The MVIIA did not promote decrease in the levels of glutamate, ROS, LP, and did not preserve the mitochondria in the intralesional application five minutes after spinal cord injury in rats.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000100150
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| Editor: |
Universidade Federal de Santa Maria
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| Relação: |
10.1590/0103-8478cr20141203
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| Formato: |
text/html
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| Fonte: |
Ciência Rural v.46 n.1 2016
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| Direitos: |
info:eu-repo/semantics/openAccess
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